Protein Purification Reagents and Kits
Proteintech Group Inc Proteintech Monoclonal Antibody, Cleaved PARP1, 1000 μg/mL, 150 μL, Clone 4G4C8
This monoclonal antibody specifically recognizes the cleaved form of PARP1, a nuclear enzyme involved in DNA damage repair. Cleaved PARP1 fragments are common biomarkers of apoptosis, resulting from caspase activation during the apoptotic process. This antibody does not recognize full-length PARP1.
- Specifically recognizes cleaved PARP1
- Clone 4G4C8
- Host species: Mouse
- Isotype: IgG1
- Reactivity: Human, mouse, rat
- Applications: Western Blot, Immunohistochemistry, Immunofluorescence/ICC, Flow Cytometry (Intra), ELISA
- Concentration: 1000 μg/mL
- Volume: 150 μL
- Formulation: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3
- Unconjugated
- Purification method: Protein G purification
- Store at -20°C; stable for one year after shipment
AURORA BIOLABS LLC TR-FRET PARP1 Trapping Assay Kit,384 rxn
PARP1 (Poly ADP-ribose Polymerase 1) is a key DNA repair enzyme that detects DNA damage and facilitates repair through PARylation, a process in which PARP1 uses NAD⁺ to add poly (ADP-ribose) chains to itself and other proteins. PARP1 activity recruits DNA repair factors to damaged sites and supports genome stability. PARP1 is frequently overexpressed in several cancers, including breast, ovarian, prostate, and lung cancers, making it an important therapeutic target. Some PARP inhibitors not only block PARP1 catalytic activity but also trap PARP1 on damaged DNA, preventing DNA repair and leading to cancer cell death, particularly in BRCA-mutant tumors. The TR-FRET PARP1 Trapping Assay Kit is designed to quantitatively measure PARP1-DNA trapping activity and evaluate compounds that modulate PARP1 binding and trapping for cancer research and drug discovery applications.
Apexbio Technology LLC Flag-tag Protein IP Assay Kit (Magnetic Beads) 50tests
Flag-tag Protein IP Assay Kit Magnetic Beads is an immunoprecipitation reagent targeting Flag-tagged fusion proteins It is designed to selectively isolate Flag-labeled proteins and their interacting complexes from complex biological samples thereby facilitating downstream biochemical and proteomic analyses Flag-tag Protein IP Assay Kit Magnetic Beads exert their activity via anti-Flag magnetic beads conjugated with monoclonal antibodies that specifically recognize the Flag epitope (DYKDDDDK peptide) enabling efficient magnetic separation Based on these properties Flag-tag Protein IP Assay Kit Magnetic Beads hold research potential in applications such as immunoprecipitation (IP) co-immunoprecipitation (Co-IP) and isolation of Flag-tagged protein complexes from cellular extracts culture supernatants serum or ascitic fluids
Zymo Research Corporation MIP Buffer, 20 ml
The MIP Buffer provides optimal conditions for the antibody/Protein A/DNA complex formation. Highlights: Sold separately or as part of the Methylated-DNA IP Kit (D5101). Storage temperature: 0°C to room temperature.
Abcam Ciglitazone, PPARgamma agonist, 50MG
MW 333.4 Da, Purity >98%. Selective PPARγ agonist (EC₅₀ = 3 μM). Shows antidiabetic and antineoplastic activity. Induces cell cycle arrest and apoptosis. Active in vivo.
The product is subject to the following: Abcam Restricted Use Statement
Medchemexpress LLC Poly(ADP-ribose) polymerase 1 (PARP1), recombinant human (Sf9, His tag) | 96.9% | 100 UG
Recombinant full-length human poly(ADP-ribose) polymerase 1 (PARP1) expressed in Sf9 insect cells with an N- or C-terminal histidine affinity tag. Supplied as a 100 μg research reagent intended for biochemical assays, enzyme activity studies, antibody validation, and structural biology.
- Recombinant full-length human PARP1 expressed in Sf9 insect cells.
- Histidine affinity tag for purification and detection.
- Packaged as 100 μg of protein suitable for research use.
- Purity 96.9% as determined by reducing SDS-PAGE.
- Suitable for biochemical assays, enzyme studies, and antibody validation.
Zymo Research Corporation ChIP DNA Clean & Concentrator, ZR-96, 2 x 96 Preps
The ZR-96 Chromatin Immunoprecipitation (ChIP) DNA Clean & Concentrator offers a rapid, high-throughput method for purifying and concentrating high-quality DNA from standard ChIP protocols, including reverse cross-linked and enzyme-digested samples. It also purifies DNA from PCR and other enzymatic reactions, making it suitable for PCR, Next-Gen sequencing (ChIP-Seq), and other molecular applications.
- Rapid high-throughput (96-well) recovery of ultra-pure DNA from chromatin immunoprecipitation (ChIP), cell lysates, Proteinase K-digested samples, PCR, and other enzymatic reactions.
- Plate design permits DNA elution at high concentrations into minimal elution volumes (> 10 μL/well).
- Eluted DNA is well-suited for use in PCR, Next-Gen sequencing (ChIP-Seq), microarrays, Southern blot analysis, and other molecular applications.
INTACT GENOMICS INC PE pegRNA Synthesis Kit 10 Reactions (100 µl RXN Volume)
The IG PE pegRNA Synthesis Kit is a perfect choice for a variety of pegRNA synthesis needs. Below is a sampling of some of the key benefits. IG PE pegRNA Synthesis Kit includes everything but your target oligos (all buffers, enzymes, scaffolds). User simply needs to provide the oligos for your target: a pair of sgRNA oligo and pegRTpbs oligo generated using the template design in this manual. Rapid Workflow (Less than 1 hour) Scalable Yield (100 µl reaction)
Abcam Cyclic Pifithrin-alpha hydrobromide, pifithrin-alpha analog, 10MG
MW 349.3 Da, Purity >98%. Stable, cell-permeable analog of pifithrin-α (ab120478). Inhibits p53-mediated apoptosis and p53-dependent gene transcription. Prevents dexamethasone-induced cell death in murine thymocytes (EC₅₀ = 2.01 μM). Potent STAT6 transcriptional inhibitor. Active in vivo and in vitro. See similar compound
The product is subject to the following: Abcam Restricted Use Statement
Bioworld DNA Denaturing Solution, 500 mL
Denaturing Solution for use in Acrylamide Gel ElectrophoresisSpecificationContains: 0.5N NaOH and 1.5M NaCl
Sigma Aldrich Fine Chemicals Biosciences Protein G Immunoprecipitation Kit sufficient for 50 assays | 1KT
Protein G Immunoprecipitation Kit sufficient for 50 assays | 1KT